Part:BBa_K1968001

Synechocystis Pcpc560 Phytobrick promoter

Pcpc560 stands for the 560bp region found upstream of the cpcB gene encoding the beta subunit of c-phycocyanin in Synechocystis sp. strain PCC 6803. This region has been reported to act as a promoter that is able of producing heterologous proteins at a level up to 15% of total soluble protein in Synechocystis, which is comparable to levels achieved in E. coli (1). In light of this, this genomic region has been termed as the Pcpc560 “super-strong” promoter. Pcpc560 contains two predicted promoters and 14 predicted transcription factor binding sites (1). A native RBS is present in the Pcpc560 sequence, meaning that this part can be cloned directly in front of a reporter (1).

(1) Zhou, J., Zhang, H., Meng, H., Zhu, Y., Bao, G., Zhang, Y., . . . Ma, Y. (2014). Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria. Sci Rep, 4, 4500. doi: 10.1038/srep04500

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 2